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KMID : 0380020080230050460
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Korean Journal of Biotechnology and Bioengineering
2008 Volume.23 No. 5 p.460 ~ p.462
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Utilization of pollen grains for the expression of epidermal growth factor
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Abstract
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Pollens grains collected from fully dehisced lily (Lilium longiflorum) anthers were given wounds by means of shaking in the presence of aluminum oxide particles. They were transformed by infiltration with Agrobacterium cells harboring a synthetic DNA encoding signal peptide-fused epidermal growth factor (EGF). After incubation for 24 hr in vitro, the pollen culture showed that EGF mRNAs and proteins were successfully expressed in the analysis of cDNA blot hybridization and immuno-blotting.
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KEYWORD
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lily pollen, agroinfiltration, epidermal growth factor, immuno-blotting
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